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KMID : 0364820130490030275
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Korean Journal of Microbiology
2013 Volume.49 No. 3 p.275 ~ p.281
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Dual Coating Improves the Survival of Probiotic Bifidobacterium Strains during Exposure to Simulated Gastro-Intestinal Conditions
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Kang Joo-Yeon
Lee Do-Kyung
Park Jae-Eun
Kim Min-Ji
Lee Joong-Su
Seo Jae-Gu
Chung Myung-Jun
Shin Hea-Soon
Ha Nam-Joo
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Abstract
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Probiotics have been reported to benefit human health by modulating immunity, lowering cholesterol, improving
lactose tolerance, and preventing some cancer. Once ingested, probiotic microorganisms have to survive harsh
conditions such as low pH, protease-rich condition, and bile salts during their passage through the gastro-intestinal
(GI) tract colonize and proliferate to exert their probiotic effects. The dual coating technology, by which the bacteria
are doubly coated with peptides and polysaccharides in consecutive order, was developed to protect the ingested
bacteria from the harsh conditions. The aim of the study was to evaluate the viable stability of a doubly coated blend
of four species of Bifidobacterium by comparing its bile/acid resistance and heat viability in vitro with that of the
non-coated blend. After challenges with acid, bile salts, heat, and viable cell counts (VVCs) of the dual coated and
non-coated blend were determined by cultivation on agar plates or flow cytometric measurement after being stain
with the BacLigtht kitTM. The results showed that the dual coated blend was much higher resistant to the acidic or
bile salt condition than the non-coated blend and heat viability was also higher, indicating that the dual coating can
improve the survival of probiotic bacteria during their transit through the GI tract after consumption.
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KEYWORD
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Bifidobacterium, dual coating technology, duolac, probiotics
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